Tag: <span>bovine serum albumin</span>

Bovine Serum Albumin Partitioning in Aqueous Two-Phase Systems: Effects of Variables and Optimization

The objective of this study was to optimize process conditions for the effective partitioning of bovine serum albumin (BSA) using response surface methodology (RSM). Initially, four different salts (tripotassium citrate, tripotassium phosphate, sodium carbonate, and sodium sulphate) were tested for the ability to partition BSA. Among the salts chosen, tripotassium citrate was observed to yield a high partition coefficient. The effect of phase forming components: concentration, PEG molecular weight, and pH were studied for a PEG/tripotassium citrate system and the information obtained was utilized to fix the ranges in RSM studies. Four different independent variables (PEG 2000, tripotassium citrate, NaCl concentrations, and pH) were considered for RSM studies and the responses generated were partition coefficient (k) and percentage yield. A statistical model was developed and the values obtained were 99% within the confidence level. Optimal conditions of the system were found as: 0.25 M sodium chloride, 32% PEG 2000 (w/w), 16% tripotassium citrate (w/w), pH 6.0, a partition coefficient of 6.03, a recovery of 91.76%, and a controlled operating temperature of 303.15 K…

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Biologics Production

biologics bioprocess bovine serum albumin bsa partitioning phase forming components response surface methodology rsm salts for partitioning

Simulation of Process Performance on Contaminant Removal Using Constant and Non-Constant Volume Diafiltration Modeling

Constant volume diafiltration (CVD) is commonly used in the biopharmaceutical industry for impurity removal or buffer exchange. The number of diavolumes is usually determined empirically or by theoretical analysis to achieve the target degree of impurity removal. There is, however, a lack of conclusive information about the effect of contaminant removal in variable volume diafiltration (VVD). VVD can occur when the diafiltration control mode is not functioning as intended. In this study, a mathematical model has been proposed to predict removal efficiency during VVD. Experiments were performed to compare the results to model calculations. A dilute concentration of bovine serum albumin solution was used as the feed solution to study variable volume effects.

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Biologics Biologics Production Bioreactor Scale-Up Cell & Gene Therapy Cell Lines Fed-Batch Bioreactor Process HEK293 Mammalian Cell Culture Manufacturing Regulatory Viral Reference Materials Viral Vectors

bovine serum albumin bsa buffer exchange constant volume diafiltration contaminant removal cvd diafiltration diafiltration modeling impurity removal permeate flow rates uf/df variable volume diafiltration vvd