ExpreS2ion, established in January 2010, is located in the Hørsholm Science Park, north of Copenhagen. It was formed as a spin-out from Affitech A/S (Affitech) which holds an equity position in the company. ExpreS2ion operates as a contract research organization (CRO) offering services related to vector and cell line development, cloning, upstream development, optimization, and production of GLP material using its S2 (Drosophila Schneider 2) cell-based ExpreS2 platform. Two of the company’s founders, Drs. de Jongh and Dyring, and early employees of ExpreS2ion were colleagues for many years at Affitech and Pharmexa A/S (Pharmexa) before the two companies combined. It was during this period that they developed and optimized a S2 expression system for use in the production of therapeutic vaccines. ExpreS2ion’s proprietary protein expression platform, ExpreS2, consists of high-yielding expression vectors, a S2 cell line that grows to higher cell densities than standard S2 cells, an optimized culture media, and a highly efficient transfection reagent specifically optimized for S2 cells…
Tag: <span>life sciences</span>
L-asparaginase, produced by Pseudomonas fluorescens, was purified by a three-phase partitioning method of t-butanol in the presence of ammonium sulphate. Enzyme recovery was enhanced by the optimization of process parameters (i.e., ammonium sulphate concentration, t-butanol ratio, temperature, and pH) using the Taguchi design of experiment (DOE) methodology. The enhanced recovery of 31.1% with 96.0% yield and purification of 10-fold was obtained…
The current global market for aptamers is approximately $99 million annually and is anticipated to increase at an astonishing compound annual growth rate (CAGR) of 106.3% for the next five years resulting in an estimated value of $3.7 billion by the year 2017. Sometimes referred to as a “synthetic antibody,” an aptamer is a nucleic acid or peptide molecule that binds to a target or antigen with high affinity and specificity. Aptamers have a wide range of applications including diagnostics, therapeutics, forensics, and biodefense. To date, hundreds of aptamer sequences have been identified and can now be chemically synthesized in the lab on demand, faster and less expensively, without the traditional issues associated with producing recombinant antibodies. This article will review aptamer technology, its advantages and limitations, as well as highlight a few of its many applications in the life sciences…
