By Daily HernĂ¡ndez, Hasel AragĂ³n, Marcos GonzĂ¡lez, Gabriela GonzĂ¡lez, Amarilis GonzĂ¡lez, AndrĂ©s Tamayo, Ivis MorĂ¡n, Pilar TĂ©llez, Eduardo SĂ¡nchez, Miguel Castillo, Williams Ferro, Camilo Ayra, and Rodolfo ValdĂ©s
Volume 15, Issue 3 (Fall 2016)
Numerous standardized techniques for detection and quantification of proteins are based on polyclonal antibody (pAb) use. However, because pAbs are a heterogeneous mixture of antibodies, there is the possibility of non-specific interactions or cross-reactions with non-related proteins, which is a disadvantage in the detection and quantification of target proteins. Therefore, the main objective of this study was to generate and characterize monoclonal antibodies (mAbs) for quantifying the Vip3Aa20 protein of Bacillus thuringiensis (Bt) expressed in event MIR162 transgenic corn plants…
Citation: HernĂ¡ndez D, AragĂ³n H, GonzĂ¡lez M, GonzĂ¡lez G, GonzĂ¡lez A, Tamayo A, MorĂ¡n I, TĂ©llez P, SĂ¡nchez E, Castillo M, Ferro W, Ayra C, ValdĂ©s R. Monoclonal antibody generation and characterization for Vip3Aa20 protein quantification in transgenic corn plants. BioProcess J, 2016; 15(3): 30–43. https://doi.org/10.12665/J153.Valdes
Posted online November 15, 2016