Two-Step Purification of Antibody from Tobacco Plants for Vaccine Manufacturing: Aqueous Two-Phase Extraction and Affinity Chromatography

By Williams Ferro, Tatiana Álvarez, Déborah Geada, Yenisley Medina, Yarysel Guevara, José Montero, Andrés Tamayo, Ariadna López, Daily Hernández, Mayra Wood, Tatiana González, Regla Somoza, and Rodolfo Valdés

Volume 14, Issue 1 (Spring 2015)

The purification of PHB-01 plantibody derived from tobacco leaves imposed difficulties when the plantibody solid-liquid extraction design was performed. Thus, our study focused on assessing a combination of an aqueous two-phase extraction (ATPE) procedure and affinity chromatography for solving some of the issues in plantibody purification. This was done using a complete factorial redesign, different polyethylene glycol (PEG)/K2PO4 proportions, and pH values in each partitioning variant…

Citation: Ferro W et al. Two-step purification of antibody from tobacco plants for vaccine manufacturing: aqueous two-phase extraction and affinity chromatography. BioProcess J, 2015; 14(1): 43–8. http://dx.doi.org/10.12665/J141.Valdes

Posted online May 1, 2015