Tag: <span>hpv</span>

The emerging use of hydrogen peroxide vapor (HPV) biodecontamination technology in the late 1990s was initially implemented as part of an alternate strategy for disinfecting rooms, areas, and whole buildings. The technology was developed with rapid cycles and was adopted by barrier isolator users allowing for rapid, effective, and validated bio-decontamination cycles inside critical zones. In the years to follow, the use of HPV technology spread throughout the development and production pipelines of small- and large-molecule biopharmaceutical products. This article will consider the current and future uses of HPV technology from the perspective of the development through manufacture of a biologically-derived product…

Biologics Production

Two cell disruption methods, mechanical and chemical, were applied for the recovery of a fusion protein named CIGB 550-E7, expressed on Escherichia coli grown in defined saline media. A comparison of the methods was done, and various operating parameters for each technique were optimized to obtain the maximum disruption efficiency and CIGB 550-E7 protein release. The mechanical disruption’s yield and recovery were 1.24 and 1.37 times higher than those obtained with chemical disruption. Modified conditions were assayed for the CIGB 550-E7 obtained by chemically defined media using the mechanical and chemical cell disruption methods.

Biologics Biologics Production Manufacturing Research