Single-use processing solutions spanning both upstream and downstream applications are being embraced by the biopharmaceutical industry. The advantages of the single-use approach to industrial cell culture versus traditional stainless steel and/or glass bioreactors has resulted in the recent commercialization of several single-use bioreactors at small (3–15L), intermediate (50–500L) and large scales (>1000L). Recent innovations are combining the features of conventional bioreactors with the ease-of-use benefits associated with single-use technology for the optimization of mammalian cell growth and recombinant protein expression. This article will provide a detailed characterization of the single-use Mobius® CellReady 3L Bioreactor capabilities as compared to a glass bioreactor, in terms of CHO cell growth, mixing, and volumetric mass transfer coefficients (kLa) for oxygen…