Optimizing Protein Expression: Producing Pure Protein for Structural Biology

by Richard Wales
Volume 3, Issue 6 (November/December 2004)


Proteins and their promise for revolutionizing drug discovery have come virtually full circle in just a few decades. The advent of genetic engineering and the emergence of early recombinant proteins such as insulin and interferon dramatically boosted the perceived value of proteins in pharmaceutical research and of protein drugs in particular. Although the lights dimmed somewhat on the promise of therapeutic proteins in subsequent years, more recent times have seen a resurgence of interest in proteins, particularly monoclonal antibodies. Perhaps most telling has been the dawn of the post-genomic era, which has cast a bright spotlight on proteins, long respected as the work-horses of the cell, for their usefulness in exploring cell function, unraveling biochemical pathways, understanding disease, and for their massive value as novel drug targets…

Citation:
Wales R. Optimizing Protein Expression: Producing Pure Protein for Structural Biology. BioProcess J, 2004; 3(6): 55-58.