by Joseph C. Fratantoni, MD, Sergey Dzekunov, PhD, Sarah Wang, and Linda N. Liu, PhD
Volume 3, Issue 6 (November/December 2004)
Across many areas of biopharmaceutical development, the goal of consistently transfecting appropriate quantities of DNA into cells has often been a significant bottleneck. Electroporation — a method of temporarily permeabilizing cell membranes by using a short electric pulse — has gained ground in recent years as an effective means of transfection. A cell loading system based on electroporation has been designed for ex vivo cell modification in a clinical setting and for incorporation into cGMP processing applications…
Citation:
Fratantoni JC, Dzekunov S, Wang S, Liu LN. A Scalable Cell-Loading System for Non-Viral Gene Delivery and Other Applications. BioProcess J, 2004; 3(6): 49-53.