by Elena M. Seletskaia, J. Paul Cowley, Michelle L. Wunderlich, Steven M. Jennings, Kenneth S. Henderson, PhD, William R. Shek, PhD, and Rajeev K. Dhawan, PhD
Volume 3, Issue 1 (January/February 2004)
Parvoviruses are one of the most prevalent infectious agents in the laboratory rodent. Their effect on research can range from immune dysfunction that may mislead researchers when interpreting results to lethal effects on animals. Until recently parvovirus infection in mice was thought to be caused by minute mouse virus (MMV) and in rats by rat viral agents in the KRV or H-1 serogroups. Relatively newly discovered viruses in these groups are mouse (MPV) and rat parvoviruses (RPV-1 and 2). Parvoviruses are 15–20 nm in diameter and are single-stranded DNA viruses of about 5,000 nucleotides, which replicate through a double-stranded DNA intermediate. The protein composition consists of three structural or capsid proteins providing the viral coat (VP-1, VP-2, and VP-3) and two non-structural proteins involved in viral replication (NS-1 and NS-2). Among the capsid proteins,VP-2 is the major protein…
Citation:
Seletskaia EM, Cowley JP, Wunderlich ML, Jennings SM, Henderson KS, Shek WR, Dhawan RK. Development of a Parvovirus Assay Using rNS-1 His-tagged Antigen. BioProcess J, 2004; 3(1): 35-39.