by Arifa S. Khan, PhD
Volume 8, Issue 3 (Fall 2009)
Cell substrates are used in various stages of viral vaccine manufacturing, as in the isolation, selection, and propagation of the virus seed or virus vector stock, as well as for the amplification of the virus to produce the final vaccine product. The various stages of cell substrate use, including cell banking, are shown in a generic manufacturing scheme in Figure 1. Traditionally, viral vaccines have been produced in animal tissues, primary cell cultures, and cell lines that either have a finite life span, such as normal diploid cells, or a theoretically infinite life span, as achieved with continuous or immortalized neoplastic cells. The cell substrates used in viral vaccines currently licensed in the US are listed in Table 1…
Citation:
Khan AS. Characterization and Qualification of Cell Substrates for Manufacturing Viral Vaccines in the United States. BioProcess J, 2009; 8(3): 8-12. http://dx.doi.org/10.12665/J83.Khan