By Nazia Mehboob, M. Javaid Asad, M. Asghar, M. Gulfraz, and Raja Tahir Mahmood
Volume 11, Issue 3 (Fall 2012)
The purpose of this study was to explore extremozyme production optimization. We found that maximum cellusases were obtained after 72 hours at 55 °C and 4.8 pH. Fructose was determined to be the optimum carbon source and peptone was the nitrogen source for cellulase production. Further, Tween 80 was used as a surfactant and ammonium sulphate served as a mediator to enhance the production of cellulases by A. fumigatus. The enzyme produced was subjected to purification by ammonium sulphate precipitation and gel filtration chromatography. The maximum concentration of cellulase was purified with 40% ammonium sulphate and gel filtration chromatography which resulted in a 3.5-fold purification. The purified enzymes were characterized with regard to optimum pH, temperature sensitivity, substrate specificity, and kinetic parameters such as Km and Vmax. Cellulase reached maximum activity at 55 °C and 5.5 pH. Metal ions like Ca2+, Mg2+, and Zn2+ had positive effects on cellulase activity. The Km and Vmax were found to be 4.34 µ M/mL and 7.29 mM respectively…
Citation: Mehboob N, Asad MJ, Asghar M, Gulfraz M, Mahmood RT. Cellulase Production by Aspergillus fumigatus Cultured on Wheat Straw Waste. BioProcess J, 2012; 11(3): 38-43. https://dx.doi.org/10.12665/J113.Mehboob
Posted online October 22, 2012