The Lentiviral Vector Reference Working Group (LVRWG) was created at the conclusion of a meeting organized by The Williamsburg BioProcessing Foundation in June 2002, in conjunction with the American Society of Gene Therapy (ASGT) annual conference. The meeting participants were gathered to evaluate the need for developing reference material to ensure comparability of lentiviral and retroviral vectors, in a similar spirit to the Adenovirus Reference Material program that had just been completed. The concensus at the conclusion of this meeting was that the diversity in the lentiviral vector field, which includes vectors derived from different parental viruses and with various designs, does not allow for identification of a single reference material that would benefit more than a single or very few investigators…
Tag: <span>replication-competent</span>
Through the tremendous efforts of the Adenovirus Reference Material Working Group (ARMWG), an adenovirus reference material (ARM) is now available from the American Type Culture Collection (ATCC). The history and progress of the ARM production and characterization has been presented at many meetings and published in numerous journal articles. Although general statements have been made regarding how the ARM should be used, there is no formal directive or specific set of instructions detailing its application in the field. The goals of this paper are (1) to briefly review the objectives for development and implementation of the ARM, (2) to describe a critical assumption necessary to meet those objectives, (3) to outline specific approaches for using the ARM, and (4) to highlight the need for a working group to address the issues raised in the process…
In the past, researchers developing gene therapy applications used replication-defective human Adenovirus 5 (Ad5) as a vector for delivering DNA sequences, almost exclusively. Ad5 vectors are typically rendered replication defective by the deletion of E1a gene sequences. A complementing cell line containing the E1a gene makes it possible to produce Ad5 vectors in large scale. Of the various cell lines that have been constructed for the purpose of high-titer Ad5 production, HEK293 cells and PER.C6 cells are the most widely used…