Incorporating a genomic regulatory sequence element can help to develop a robust expression vector, leading to improved recombinant protein expression in cultured cells. Such elements include the Expression Augmenting Sequence Element (EASE), scaffold- or matrix-attachment regions (S/MAR elements), Insulators, or the Universal Chromatin Opening Element (UCOE). However, generating a robust vector further requires having the relevant vector components in a context-optimized manner…