by Jennitte Stevens, John Laudemann, Jackie Sheng, Michelle Wu, and Helen Kim
Volume 7, Issue 4 (Winter 2008)
Incorporating a genomic regulatory sequence element can help to develop a robust expression vector, leading to improved recombinant protein expression in cultured cells. Such elements include the Expression Augmenting Sequence Element (EASE), scaffold- or matrix-attachment regions (S/MAR elements), Insulators, or the Universal Chromatin Opening Element (UCOE). However, generating a robust vector further requires having the relevant vector components in a context-optimized manner…
Citation:
Stevens J, Laudemann J, Sheng J, Wu M, Kim H. Expression Vector Engineering for Protein Production in Mammalian Cells. BioProcess J, 2008; 7(4): 38-42. http://dx.doi.org/10.12665/J74.Kim