Microbial fermentation is used to produce such products as therapeutic proteins, antibiotics, hormones, enzymes, amino acids, blood substitutes and alcohol. These products may be expressed by the microbial cells themselves, expressed intracellularly in the cytoplasm or, in the case of bacterial cells, in the periplasmic material. Before any intracellularly-produced molecules can be used and further purified, the cells (i.e., E. coli) must be concentrated and set in an appropriate buffer (cell washing) before being lysed. Then after lysis, molecules of interest have to be separated from the parent cells and then clarified to remove cellular debris and other contaminants. Depending on the process used, additional clarification steps may be necessary…
Tag: <span>concentration</span>
Adenoviral vectors (AAV’s) offer a promising new approach to vaccine development. They have the ability to be rapidly manipulated for bearing transgenic coding for specific antigenic proteins, efficiently infect a variety of mammalian cell types (including antigen-presenting cells) and induce a broad immune response against the target antigen in vaccine recipients. Furthermore, AAV’s offer an excellent safety profile, in that they can be engineered to be non-replicating in the vaccine recipient and they lack the molecular mechanism for integration into the host genome. AAV’s are highly amenable to scalable manufacturing processes such as the use of stirred tank bioreactors, high capacity filtration methods, and chromatographic purification procedures…