Tangential Flow Filtration Membranes For the Washing of Escherichia coli Cells

by Geraldine Eschbach and Steve Vermant
Volume 8, Issue 1 (Spring 2009)

Microbial fermentation is used to produce such products as therapeutic proteins, antibiotics, hormones, enzymes, amino acids, blood ­substitutes and ­alcohol. These products may be expressed by the microbial cells themselves, expressed intracellularly in the cytoplasm or, in the case of bacterial cells, in the periplasmic material. Before any intracellularly-produced molecules can be used and further purified, the cells (i.e., E. coli) must be concentrated and set in an ­appropriate buffer (cell washing) before being lysed. Then after lysis, molecules of ­interest have to be separated from the ­parent cells and then clarified to remove ­cellular debris and other contaminants. Depending on the process used, additional clarification steps may be necessary…

Citation:
Eschbach G, Vermant S. Tangential Flow Filtration Membranes For the Washing of Escherichia Coli Cells. BioProcess J, 2009; 8(1): 46-48. http://dx.doi.org/10.12665/J81.EschbachVermant