Profiling Intracellular Monoclonal Antibody Expression for Improved Cell Culture Characterization and Process Optimization

by Matthew S. Manahan and Wai Lam W. Ling, PhD
Volume 8, Issue 3 (Fall 2009)

Many laboratories have utilized cell-free systems or prokaryotic systems designed to produce biological molecules with single polypeptide chains, limited folding requirements, and without glycosylation. The yeast systems are used to generate glycoproteins; however, their glycosylation profiles are vastly different from those of mammalian cells. Without significant glycoengineering, the yeast-produced recombinant glycoproteins may be unsuitable as therapeutic molecules. As such, the use of mammalian cells is still the preferred method to produce complex biological molecules…

Citation:
Manahan MS, Ling WLW. Profiling Intracellular Monoclonal Antibody Expression for Improved Cell Culture Characterization and Process Optimization. BioProcess J, 2009; 8(3): 32-37. http://dx.doi.org/10.12665/J83.Ling