Long-Term CB.Hep-1 Monoclonal Antibody Production in a Single-Use Bioreactor on a Rocker Platform with Serum- and Protein-Free Media

By Alberto Galván, Rodolfo Valdés, Marcos González, Hasel Aragón, Daily Hernández, Sigifredo Padilla, Leonardo Gómez, David Gailán, Yanet Villegas, Andrés Tamayo, Adelma Pérez, Bárbara Pérez, Maylín Lao, Yodelis Calvo, Aneet Fernández, Lianys Lee, Jania Suarez, Judey García, and Eduardo Ojito

Volume 14, Issue 4 (Winter 2015/2016)

Rocker bag bioreactors have been used successfully in cultivating cells because they provide good nutrient distribution and cell suspension while eliminating the need to validate cleaning and sterilization. Therefore, this study examined the long-term performance of a 50 L single-use bag bioreactor on a rocking platform in CB.Hep-1 monoclonal antibody (mAb) production. For such a purpose, the bioreactor was operated in a continuous mode with a mixture of serum-free media (SFM) for 62 days, and with protein-free medium (PFM) for another 62 days…

Citation: Galván A, ValdĂ©s R, González M, AragĂłn H, Hernández D, Padilla S et al. Long-term CB.Hep-1 monoclonal antibody production in a single-use bioreactor on a rocker platform with serum- and protein-free media. BioProcess J, 2016; 14(4): 4–13. http://dx.doi.org/10.12665/J144.Valdes

Posted online January 12, 2016

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Biologics Biologics Production Bioreactor Scale-Up Research

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