Bio-Electrospraying Primary Neonatal Cardiac Myocytes

by Seán P. Barry, Suwan N. Jayasinghe, PhD, David S. Latchman, and Anastasis Stephanou
Volume 6, Issue 1 (Spring 2007)

Controlled cell deposition by way of micrometer-sized jets are increasingly becoming a fiercely pursued area of research. We recently uncovered the ability to jet living cells using one such jetting methodology, now referred to as bio-electrosprays. This technique has never been explored for processing living cells until now. Electrosprays charge media within a needle, subsequently imposing an external electric field to assist in drawing the media into a micro-jet. The resulting instabilities assist in jet break-up, forming cell-bearing droplets. These droplets containing viable cells could be deposited onto a wide variety of biological and non-biological substrates. In this article, we report our developmental studies into this jet approach with a view to the successful handling and deposition of primary neonatal cardiac myocytes…

Barry SP, Jayasinghe SN, Latchman DS, Stephanou A. Bio-Electrospraying Primary Neonatal Cardiac Myocytes. BioProcess J, 2007; 6(1): 8-14.