by Barry J. Sugarman, PhD, Beth M. Hutchins, PhD, Diane L. McAllister, Fei Lu, MD, and Kenneth B. Thomas, PhD
Volume 2, Issue 5 (September/October 2003)
The Adenovirus Reference Material Working Group (ARMWG) oversaw development of an adenovirus reference material (ARM) with the intent to provide a way to standardize assay measurements from different laboratories. The ARM, which was manufactured in stages by various organizations including Canji (San Diego, CA) and Introgen Therapeutics (Houston, TX), is available from American Type Culture Collection (Manassas, VA). Upon completion of its manufacture, the characterization phase primarily defined viral particle concentration as well as infectious titer for this product. However, many other concurrent characterization studies were conducted including an assessment of vector purity (e.g., host cell DNA, host cell protein, reversed-phase HPLC), a short-term field use and shipping stability study, and a long-term stability study. Also included in these studies was a coordinated effort to determine the complete DNA sequence of the ARM vector genome…
Citation:
Sugarman BJ, Hutchins BM, McAllister DL, Lu F, Thomas KB. The Complete Nucleic Acid Sequence of the Adenovirus Type 5 Reference Material (ARM) Genome. BioProcess J, 2003; 2(5): 27-33.