Tag: <span>amino acid detection</span>

The quantitative and qualitative analysis of proteins and their amino acid sequence composition is a critical operation in many research laboratories and operations. Much protein analysis is performed using high-pressure liquid chromatography (HPLC) with ultraviolet (UV) or fluorescence detection. Although these methods are robust and widely used, certain issues limit their utility. For example, some proteins or peptides may have a poor UV response and can be difficult to detect. Direct comparisons of protein levels when quantitated directly by UV can also be problematic due to differences in extinction coefficients of various proteins…