By Pete Gagnon, Blaž Gori?ar, Sara Drmota Prebil, Hana Jug, Maja Leskovec, and AleŔ Štrancar
Volume 20, Open Access (Oct 2021)
Separation of empty and full AAV8 capsids was achieved during their elution from a weak anion exchanger with an ascending pH gradient at low conductivity. Experimental data suggest elution was mediated by loss of positive charge from the exchanger. The method produced a full capsid peak with fewer empty capsids than elution of a strong anion exchanger with a salt gradient. Elution of the weak exchanger by sodium chloride gradients or by pH gradients in the presence of sodium chloride gave inferior separation performance. Pre-elution of empty capsids with a pH step allowed full capsids to be eluted by salt without compromising separation. Loading at intermediate pH prevented empty capsid binding and enabled step elution of full capsids in a physiological buffer environment.
Citation:
Gagnon P, Gori?ar B, Drmota Prebil S, Jug H, Leskovec M, Å trancar A. Separation of empty and full adeno-associated virus capsids from a weak anion exchanger by elution with an ascending pH gradient at low ionic strength. BioProcess J, 2021; 20.
https://doi.org/10.12665/J20OA.Gagnon
Posted online October 11, 2021.