Large-Scale Chromatographic Purification of an Attenuated Chimeric Poliovirus

by Tom Ouellette, Steven L. Giardina, PhD, Earl Nelson, Judith Poiley-Nelson, PhD, Trevor Broadt, and Sibylle Herzer, PhD
Volume 4, Issue 2 (March/April 2005)


Poliovirus is a small (28-30 nm diameter) non-enveloped RNA virus belonging to the family Picornaviridae. The ability of poliovirus to cross the blood-brain barrier and its natural infectivity of central nervous system (CNS) tissue via the CD155 receptor, found exclusively in primates, has promoted the investigation of an attenuated poliovirus for the treatment of malignant gliomas. However, use of the virus in clinical testing is limited due to low yields obtained from conventional purification methodologies…

Citation:
Ouellette T, Giardina SL, Nelson E, Poiley-Nelson J, Broadt T, Herzer S. Large-Scale Chromatographic Purification of an Attenuated Chimeric Poliovirus. BioProcess J, 2005; 4(2): 31-38.