by Michel Pailhes, Charles Lambalot, and Robert Barloga
Volume 3, Issue 3 (May/June 2004)
One of the biggest challenges in the production of recombinant therapeutic proteins, monoclonal antibodies, and vaccines is the clarification and separation of the product (typically a protein) from the cell culture or fermentation broth. The desired product is present in low concentrations and must be efficiently separated from the other components present in the bioreactor fluid. An overall objective in developing a clarification process is to achieve the highest level of product recovery (yield) and contaminant removal with the fewest number of unit processes. Understanding how each operational step affects the performance of the next step downstream is the challenge at hand. Centrifugation, in combination with depth filtration, is gaining acceptance as the preferred method for the removal of cells, cell debris, colloids, insoluble precipitants, aggregates, and other materials found in mammalian cell culture and bacterial fermentation fluids…
Citation:
Pailhes M, Lambalot C, Barloga R. Integration of Centrifuges with Depth Filtration for Optimized Cell Culture Fluid Clarification Processes. BioProcess J, 2004; 3(3): 55-58.