by Warren Schwartz, PhD, Jin-an Jiao, Jill Ford, Daniel Conrad, Jean-Francois Hamel, Patrick Santambien, Lisa Bradbury, and Thibault Robin
Volume 3, Issue 5 (September/October 2004)
A growing number of separationsā scientists and process developers are looking beyond protein A sorbents for capture and initial purification of monoclonal antibodies. A variety of strategic and operational goals have prompted examination of alternative immunoglobulin-selective sorbents. Most broadly, many workers wish to eliminate design considerations associated with leached protein A. Also cited is a preference for sorbents that can withstand stringent cleanup using 1 M sodium hydroxide. In some applications, it is desirable to avoid the low-pH elution conditions typically employed with protein A sorbents ā conditions that can foster aggregate formation. In still other cases, the target antibody may bind poorly to protein A. Finally, there may be interest in evaluation of immunoglobulin-selective sorbents less costly than protein A sorbents…
Citation:
Schwartz W, Jiao J, Ford J, Conrad D, Hamel J, Santambien P, Bradbury L, Robin T. Application of Chemically-Stable Immunoglobulin-Selective Sorbents: Capture and Purification of Antibodies with Resolution of Aggregate. BioProcess J, 2004; 3(5): 53-62.