by Erik J. Bonten and Alessandra d’Azzo
Volume 5, Issue 3 (Fall 2006)
Baculovirus infection of insect cells is an established method to obtain large quantities of biologically active recombinant proteins with properties similar to those of proteins expressed in mammalian cells. Insect cells are capable of most mammalian posttranslational modifications that control protein compartmentalization, secretion, targeting to nucleus or cell surface, N- and O-glycosylation, phosphorylation, proteolytic processing, and assembly of multi-protein complexes. The baculovirus transfer plasmids and accessory products utilized for protein expression in insect cells are currently available from several commercial sources. The plasmids often contain his-tags to facilitate Ni-NTA purification of recombinant proteins, and/or signal peptides to promote or enhance secretion of the proteins into the medium. Typically, in most baculovirus cloning vectors the coding region of the polyhedrin gene has been replaced by a polylinker with multiple cloning sites for the insertion of the cDNA of interest downstream of the strong polyhedrin promoter…
Citation:
Bonten EJ, d’Azzo A. Plaque Assay of Recombinant Baculovirus Using Commercially Available Serum-Free Insect Cell Medium: Improved Plaque Morphology, Easy Detection, and cGMP Compatibility. BioProcess J, 2006; 5(3): 35-37. https://doi.org/10.12665/J53.Bonten