by Robert Mattes et al.
Volume 8, Issue 4 (Winter 2009/2010)
Viable cell density (VCD), the quantitative assessment of living cells, is commonly determined by laborious and inaccurate off-line cell counting methods. Single-function in situ probes have been developed using various technologies including optical density, radio frequency and dielectric permittivity. Optical density measurements predict total biomass but are sensitive to cell debris accumulation and inherent media turbidity. Near-infrared (NIR) has the advantage of being able to measure many key analytes in the cell culture simultaneously. NIR has been used to acquire real-time measurements of glucose, glutamine, glutamate, and media nutrients such as amino acids and metabolites (e.g., lactate and ammonia). NIR probes can be sterilized in place, and real-time measurements can be acquired throughout the mammalian cell culture processes…
Citation:
Mattes R et al. Monitoring Viable Cell Density in Bioreactors Using Near-Infrared Spectroscopy. BioProcess J, 2009; 8(4): 38-41. http://dx.doi.org/10.12665/J84.Sugui