by Vladimir Slepushkin, MD, PhD, Nancy Chang, Reuben Cohen, Yuxiang Gan, Bing Jiang, Eden Deausen, David Berlinger, Gwendolyn Binder, PhD, Kris Andre, Laurent Humeau, PhD, and Boro Dropulic, PhD
Volume 2, Issue 5 (September/October 2003)
The use of virus-based vectors for gene transfer has become an important delivery method for both in vitro applications and in vivo experimental clinical therapies. In small-scale experimental applications, most vectors can easily be concentrated and purified by simple methods (for example, ultracentrifugation.) However, it is challenging to scale up centrifugation-based vector purification methods for the large-scale production required for clinical use. In particular, when considering production of vector for human use, additional steps such as final sterilization by filtration must be taken to ensure the purity and safety of the vector preparation. Because the vector aggregates when pelleted by centrifugation, sterile filtration will eliminate vector particles from the solution. An efficient vector purification process that maintains vector potency is an important step in vector production for gene therapy…
Citation:
Slepushkin V, Chang N, Cohen R, Gan Y, Jiang B, Deausen E, Berlinger D, Binder G, Andre K, Humeau L, Dropulic B. Large-scale Purification of a Lentiviral Vector by Size Exclusion Chromatography or Mustang Q Ion Exchange Capsule. BioProcess J, 2003; 2(5): 89-95.