In Situ Hybridization: The Importance of Ultrapure Water for RNA Technologies

by Frauke Nitzki and Elmar Herbig
Volume 13, Issue 4 (Winter 2014/2015)

In situ hybridization (ISH) for localization of DNA/RNA hybrids in cytological preparations was first described in 1969 by Gall and Pardue. This method enables mRNA transcripts to be detected in tissue sections. Unlike expression analyses based on polymerase chain reactions, the exact localization of the target transcripts can be identified within the tissue...

Nitzki F, Herbig E. In situ hybridization: the importance of ultrapure water for RNA technologies. BioProcess J, 2015; 13(4): 56–9.

Posted online January 20, 2015.