Direct, Real-Time Antibody-Based Quantification of Baculovirus

by Michael Artinger, April Birch, Tyler Gates, Christopher Kemp, and Michael W. Olszowy
Volume 14, Issue 4 (Winter 2015/2016)

The impact of viruses—in geopolitical human health issues, in the production of vaccines and recombinant proteins, and in gene therapy and cancer treatments—highlights the need for a better understanding of the systems that are dependent upon them. A primary barrier to recognizing the full potential of these life-saving biomedical approaches is the scarcity of analytical methods capable of providing biologically relevant information without hindering the pace of development and production. ViroCyt® is a Colorado-based biotechnology company with one overriding focus: Enabling the rapid and specific quantification of viruses and virus-related particles. The ViroCyt Virus Counter® was designed to meet this objective. Until recently, the primary method employed by the Virus Counter for detecting virus was ComboDye, which utilized a general fluorogenic nucleic acid stain and a general fluorogenic protein stain to simultaneously label intact virus particles. The advantage of this approach is that it provides a broadly applicable reagent capable of quantifying many virus types. The disadvantage is that crude and/or complex matrices, in which there is high nucleic acid and/or protein background, can complicate the detection of individual virus particles. To address this issue, and to allow innovation to drive the technology into an expanded number of virus systems, ViroCyt recently created a second portfolio of reagents called ViroTag®. This technology employs fluorescently tagged antibodies specific for the virus of interest. The first members of this product line to be launched target two high-value viruses: baculovirus and human adenovirus. In this article, we focus on the application of the ViroTag system for rapid quantification during the bioprocessing of baculovirus-derived systems...

Artinger M, Birch A, Gates T, Kemp C, Olszowy MW. Direct, real-time antibody-based quantification of baculovirus. BioProcess J, 2016; 14(4): 15–21.

Posted online January 12, 2016.