Design and Optimization of a Purification Process for MY32/Ls Protein Solubilizing Inclusion Bodies for a New Vaccine Against Sea Lice

by Carlos Perez Heredia, Nemecio González Fernández, Eladio Salazar Gómez, Eulogio Pimentel Vázquez, Yamila Carpio González, and Miladys Limonta Fernández
Volume 14, Issue 1 (Spring 2015)

Sea lice are the most problematic marine pathogens the salmon industry has to deal with, significantly affecting Europe and America. The worst offenders are genera: Pseudocaligus, Caligus, and Lepeophtheirus. Over €305 million in losses are estimated. Recent results have suggested that subolesin/akirin/myosin32 are good candidate antigens for the control of arthropod infestations such as sea lice. The aim of this study was to design and optimize the purification step of MY32/Ls protein to obtain the active pharmaceutical ingredient against sea lice. Non-chromatographic purification strategies were employed, based on published works, to establish rupture, washing, solubilization, and refolding conditions...

Heredia CP, Fernández NG, Gómez ES, Vázquez EP, González YC, Fernández ML. Design and optimization of a purification process for MY32/Ls protein solubilizing inclusion bodies for a new vaccine against sea lice. BioProcess J, 2015; 14(1): 49–59.

Posted online May 1, 2015.