Successful High Density Escherichia coli Fermentation Using the Eppendorf BioFlo® 320 Advanced Bioprocess Control System

by Bin Li, PhD and Ma Sha, PhD
Volume 14, Issue 1 (Spring 2015)

The gram-negative bacterium, Escherichia coli, has a long history in the world of laboratory and industrial processes due to its ease of manipulation and well-understood genome. It is widely cultured under aerobic conditions. High cell density cultivation of E. coli is a powerful technique for the production of recombinant proteins. Indeed, 30% of the FDA-approved biopharmaceuticals on the market are produced in E. coli. An Escherichia coli fermentation run conducted using the Eppendorf BioFlo® 320 bioprocess control station achieved high cell density at 12 hours, as determined by a maximum optical density (OD600) measurement of 215.2. The weights of dry and wet cells were also measured...

Li B, Sha M. Successful high density Escherichia coli fermentation using the Eppendorf BioFlo® 320 advanced bioprocess control system. BioProcess J, 2015; 14(1): 20–4.

Posted online May 1, 2015.