Impact of Process Loading on Optimization and Scale-Up of TFF Microfiltration

by Seat Yee Lau, Priyabrata Pattnaik, PhD, Takao Ito, PhD, and Bala Raghunath, PhD
Volume 13, Issue 2 (Summer 2014)

Tangential flow filtration (TFF) microfiltration has been used as one of the choices for clarification of mammalian cell or microbial cell culture in the biopharmaceutical industry. Unlike the ultrafiltration process for protein concentration and the diafiltration application where the feed solution is relatively clean (free of colloids or larger particles after the clarification/purification process), the microfiltration process needs to handle a rather high-fouling feed stream such as cells, cell debris, colloids, etc. In a previously published article, we discussed that a TFF microfiltration step is limited by a maximum throughput or capacity obtainable under a given set of operating conditions. Some distinct microfiltration characteristics, such as critical permeate flux, permeate flux control, and maximum throughput were explained in that article...

Lau SY, Pattnaik P, Ito T, Raghunath B. Impact of process loading on optimization and scale-up of TFF microfiltration. BioProcess J, 2014; 13(2): 46–55.

Posted online July 10, 2014.