Use of Small-Scale, Single-Use Bioreactors for Streamlining Upstream Process Development

by Amy Kittredge Wood, Shrikanth Gowda, Laura Dinn, Janice Simler, Jane Ring, John Mumira, Michael Cunningham, and Peter J. Rapiejko
Volume 10, Issue 1 (Summer 2011)

Single-use processing solutions spanning both upstream and downstream applications are being embraced by the biopharmaceutical industry. The advantages of the single-use approach to industrial cell culture versus traditional stainless steel and/or glass bioreactors has resulted in the recent commercialization of several single-use bioreactors at small (3–15L), intermediate (50–500L) and large scales (>1000L). Recent innovations are combining the features of conventional bioreactors with the ease-of-use benefits associated with single-use technology for the optimization of mammalian cell growth and recombinant protein expression. This article will provide a detailed characterization of the single-use Mobius® CellReady 3L Bioreactor capabilities as compared to a glass bioreactor, in terms of CHO cell growth, mixing, and volumetric mass transfer coefficients (kLa) for oxygen...

Citation:
Kittredge Wood A, Gowda S, Dinn L, Simler J, Ring J, Mumira J, Cunningham M, Rapiejko PJ. Use of Small-Scale, Single-Use Bioreactors for Streamlining Upstream Process Development. BioProcess J, 2011; 10(1): 34-39. http://dx.doi.org/10.12665/J101.Wood.