by A.H. McIntosh, PhD, J.J. Grasela, and C.L. Goodman
Volume 5, Issue 2 (Summer 2006)
Baculoviruses have found many uses in the field of biology, including as a control strategy for major insect pests such as the gypsy moth, the corn earworm, and the velvetbean caterpillar, for high level expression of recombinant proteins in insect cells, and, more recently, as gene delivery vehicles into mammalian cells. Some of these uses entail the need for isolation of baculoviral DNA for molecular biology studies. Currently, there are three ways in which baculoviral DNA can be obtained: 1) from the infection of insect larvae or cell cultures and the recovery of occlusion bodies (OB) containing viral particles, from which the DNA is then extracted; 2) from the recovery of extracellular virus (ECV) or budded virus (BV), usually from infected cell cultures; or 3) from total intracellular DNA harvested from infected cell cultures early in the infection cycle…
Citation:
McIntosh AH, Grasela JJ, Goodman CL. A Simplified and Rapid Method for Extraction of DNA from Baculovirus Occlusion Bodies.BioProcess J, 2006; 5(2): 59-61. https://doi.org/10.12665/J52.McIntosh