Antibody-Dependent Cellular Phagocytosis: The Mechanism of Action That Gets No Respect
A Discussion About Improving Bioassay Reproducibility

by Ulrike Herbrand, PhD
Volume 15, Issue 1 (Spring 2016)

Antibody-dependent cellular phagocytosis (ADCP), which relies on macrophages to attack and devour tumor cells following antibody binding, is a potentially useful mechanism of action (MOA) for antibody drug developers and vaccine makers to consider in determining product efficacy. Unfortunately, it is often ignored in favor of more accessible MOAs driving biological function such as antibody-dependent cellular cytotoxicity (ADCC) because the assays are tedious to prepare, perform, and reproduce. This article will summarize a number of those challenges and discuss how novel approaches, such as a reporter-gene assay that replaces the macrophages with an engineered cell line, are more reliable in measuring this neglected MOA. We will also discuss why this could help developers better understand how their biosimilars compare to the originator product...

Herbrand U. Antibody-dependent cellular phagocytosis: the mechanism of action that gets no respect — A discussion about improving bioassay reproducibility. BioProcess J, 2016; 15(1): 26–9.

Posted online April 7, 2016.